THP-1-CAS9 cell line

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the THP-1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a THP-1-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
THP-1-CAS9	Cas9	18.14743614	4.86366844
THP-1-CAS9	β-actin	13.28376770	4.86366844
THP-1	Cas9	34.46055222	21.80498600
THP-1	β-actin	12.65556622	21.80498600

Cutting Efficiency Validation

THP-1-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the THP-1-CAS9 stable cell pool which is electroporated by UBA7 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:THP-1			STR Info (Cell bank) Cell Line:THP-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13	13		13	13
D7S820	10	10		10	10
D16S539	11	12		11	12
VWA	16	16		16	16
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	11	13		11	13
D12S391	19	19
FGA	24	25
D2S1338	17	18
D21S11	30	31.2
D18S51	13	14
D8S1179	10	14
D3S1358	15	17
D6S1043	14	14
PENTAE	11	15
D19S433	12.2	13
PENTAD	10	12
D1S1656	16	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

THP-1-CAS9

Catalog#

YC-D011-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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THP-1-CAS9 cell line

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USP20 Knockout cell line (THP-1)
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ALDH3A2 Knockout cell line (THP-1)
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STX8 Knockout cell line (THP-1)
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